Storm Crow
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This just came out. As if we didn't have enough problems!
Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production
https://www.mdpi.com/1999-4915/11/9/802
Viruses 2019, 11(9), 802; Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production
Received: 5 August 2019 / Revised: 27 August 2019 / Accepted: 29 August 2019 / Published: 29 August 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract
In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV. View Full-Text
Keywords: Closteroviridae; Crinivirus; Bemisia tabaci; chlorosis; shoot propagation
There were photos of affected leaves, but for some reason they won't post.
Granny
Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production
https://www.mdpi.com/1999-4915/11/9/802
Viruses 2019, 11(9), 802; Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production
Received: 5 August 2019 / Revised: 27 August 2019 / Accepted: 29 August 2019 / Published: 29 August 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text Download PDF
Abstract
In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV. View Full-Text
Keywords: Closteroviridae; Crinivirus; Bemisia tabaci; chlorosis; shoot propagation
There were photos of affected leaves, but for some reason they won't post.
Granny
